BS ISO 20397-1:2022

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Biotechnology. Massively parallel sequencing – Nucleic acid and library preparation

Published By	Publication Date	Number of Pages
BSI	2022	24

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Description

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PDF Pages	PDF Title
2	undefined
7	Foreword
8	Introduction
9	1 Scope 2 Normative references 3 Terms and definitions
10	4 Nucleic acid sample quality evaluation 4.1 General
11	4.2 Sample quantification 4.3 Sample purity 4.4 Sample integrity 4.4.1 General 4.4.2 Agarose gel electrophoresis 4.4.3 Capillary gel electrophoresis 4.4.4 Microfluidic analysis system 4.4.5 PCR method
12	5 Nucleic acid library preparation 5.1 General 5.2 Fragmentation 5.2.1 General 5.2.2 Mechanical fragmentation 5.2.3 Enzymatic fragmentation
13	5.2.4 Chemical fragmentation 5.2.5 Fragmented nucleic acid sample quantity 5.2.6 Fragmented nucleic acid sample purity 5.2.7 Fragmented nucleic acid size distribution 5.2.8 Fragmented nucleic acid purification using gel electrophoresis 5.3 Addition of universal sequences 5.3.1 Repair 5.3.2 Ligation of adapter
14	5.3.3 Barcoding/indexing 5.4 Size selection 5.5 Amplification 5.6 Purification and clean up procedures
15	5.7 Library quantification 5.7.1 Library quantification method 5.7.2 Selection of quantification method 5.8 Library qualification 5.8.1 General 5.8.2 Methods 6 Validation
16	7 Reference materials or controls 7.1 General 7.2 Control samples 7.3 Positive control
17	7.4 Negative control 7.5 No-template control 7.6 Spike-in control 7.7 Reference materials 8 Contaminations 8.1 General 8.2 Primary sample evaluation
18	8.3 Protocol and operation procedure
19	Annex A (informative) Checklist for sample quality assessment before library construction
20	Annex B (informative) Examples of quality criteria for selected MPS platforms and applications
22	Annex C (informative) Reference material list
23	Bibliography

Additional information

Status	Definitive
Pages	24
Publication Date	2022-05-16
ISBN	978 0 539 05389 0
Standard Number	BS ISO 20397-1:2022
Title	Biotechnology. Massively parallel sequencing – Nucleic acid and library preparation
Identical National Standard Of	ISO 20397-1
Descriptors	Biotechnology, Immobilization (biotechnology), Connection in parallel, Nitrogen heterocyclic aromatic compounds, Nucleic acids, Nucleic acids
Publisher	BSI
Committee	BTI/1
ICS Codes	07.080 - Biology. Botany. Zoology

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BS ISO 20397-1:2022

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