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BS ISO 7251:2005+A1:2023

$142.49

Microbiology of food and animal feeding stuffs. Horizontal method for the detection and enumeration of presumptive Escherichia coli. Most probable number technique

Published By Publication Date Number of Pages
BSI 2023 24
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PDF Catalog

PDF Pages PDF Title
2 National foreword
7 Foreword
8 Introduction
9 1 Scope
2 Normative references
10 3 Terms and definitions
4 Principle
4.1 Detection method
4.2 Enumeration method
11 5 Diluent, culture media and reagent
5.1 Diluent
5.2 Selective enrichment medium (lauryl sulfate broth)
5.2.1 Composition
12 5.2.2 Preparation
5.3 EC broth (selective medium)
5.3.1 Composition
5.3.2 Preparation
5.3.3 Performance testing and quality assurance of the culture medium
5.4 Peptone water, indole free
5.4.1 Composition
5.4.2 Preparation
13 5.5 Indole reagent (Kovacs’ reagent)
5.5.1 Composition
5.5.2 Preparation
5.5 Performance testing
14 6 Apparatus and glassware
6.1 Apparatus for dry sterilization (oven) or wet sterilization (autoclave)
15 7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Detection method
9.1.1 Test portion and initial suspension
9.1.2 Incubation of the selective enrichment medium (lauryl sulfate broth)
9.1.3 Inoculation and incubation of the selective medium (EC broth)
16 9.1.4 Inoculation and incubation of the peptone water
9.1.5 Examination for indole production
9.1.6 Interpretation
9.2 Enumeration method
9.2.1 Test portion, initial suspension and dilutions
9.2.2 Inoculation and incubation of the selective enrichment medium (lauryl sulfate broth)
17 9.2.3 Subculturing and incubation of the selective medium (EC broth)
9.2.4 Inoculation and incubation of the peptone water
9.2.5 Examination for indole production
9.2.6 Interpretation
10 Expression of results
10.1 Detection method
10.2 Enumeration method
11 Test report
19 Annex A (normative) MPN table
22 Bibliography
BS ISO 7251:2005+A1:2023
$142.49