BS ISO 7251:2005+A1:2023
$142.49
Microbiology of food and animal feeding stuffs. Horizontal method for the detection and enumeration of presumptive Escherichia coli. Most probable number technique
Published By | Publication Date | Number of Pages |
BSI | 2023 | 24 |
PDF Catalog
PDF Pages | PDF Title |
---|---|
2 | National foreword |
7 | Foreword |
8 | Introduction |
9 | 1 Scope 2 Normative references |
10 | 3 Terms and definitions 4 Principle 4.1 Detection method 4.2 Enumeration method |
11 | 5 Diluent, culture media and reagent 5.1 Diluent 5.2 Selective enrichment medium (lauryl sulfate broth) 5.2.1 Composition |
12 | 5.2.2 Preparation 5.3 EC broth (selective medium) 5.3.1 Composition 5.3.2 Preparation 5.3.3 Performance testing and quality assurance of the culture medium 5.4 Peptone water, indole free 5.4.1 Composition 5.4.2 Preparation |
13 | 5.5 Indole reagent (Kovacs’ reagent) 5.5.1 Composition 5.5.2 Preparation 5.5 Performance testing |
14 | 6 Apparatus and glassware 6.1 Apparatus for dry sterilization (oven) or wet sterilization (autoclave) |
15 | 7 Sampling 8 Preparation of test sample 9 Procedure 9.1 Detection method 9.1.1 Test portion and initial suspension 9.1.2 Incubation of the selective enrichment medium (lauryl sulfate broth) 9.1.3 Inoculation and incubation of the selective medium (EC broth) |
16 | 9.1.4 Inoculation and incubation of the peptone water 9.1.5 Examination for indole production 9.1.6 Interpretation 9.2 Enumeration method 9.2.1 Test portion, initial suspension and dilutions 9.2.2 Inoculation and incubation of the selective enrichment medium (lauryl sulfate broth) |
17 | 9.2.3 Subculturing and incubation of the selective medium (EC broth) 9.2.4 Inoculation and incubation of the peptone water 9.2.5 Examination for indole production 9.2.6 Interpretation 10 Expression of results 10.1 Detection method 10.2 Enumeration method 11 Test report |
19 | Annex A (normative) MPN table |
22 | Bibliography |