BSI PD CEN/TS 15634-3:2016
$102.76
Foodstuffs. Detection of food allergens by molecular biological methods – Hazelnut (Corylus avellana). Qualitative detection of a specific DNA sequence in chocolate by real-time PCR
| Published By | Publication Date | Number of Pages |
| BSI | 2016 | 18 |
This Technical Specification describes a procedure for the qualitative detection of hazelnut (Corylus avellana) in chocolate. DNA is extracted from the chocolate and a specific DNA sequence for hazelnut detected from the gene for corA 1 [ 4], [ 5].
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 4 | Contents Page |
| 5 | European foreword |
| 6 | 1 Scope 2 Principle 3 Reagents 3.1 DNA extraction with CTAB |
| 7 | 3.2 DNA purification by means of solid phase extraction 3.3 Real-time PCR reagents |
| 8 | 4 Apparatus and equipment 4.1 DNA extraction 4.2 PCR 5 Procedure 5.1 General 5.2 Sample preparation |
| 9 | 5.3 DNA extraction with CTAB 5.4 DNA purification by means of solid phase extraction |
| 10 | 5.5 Measuring the mass concentration of the extracted DNA and setting to target concentration 5.6 Real-time |
| 11 | Table 1 — Reaction batch for the real-time PCR Table 2 — Temperature/time program when using a light cycler |
| 12 | Table 3 — Temperature/time program when using a block cycler 6 Validation status and performance criteria 6.1 General 6.2 Detection |
| 13 | 6.3 Reliability of the method 6.3.1 Setup of the interlaboratory study 6.3.2 Results Table 4 — Overview data for the interlaboratory study |
| 14 | Table 5 — Number of PCR reactions showing false-negative or false-positive results 6.3.3 Specificity 7 Test report |
| 15 | Bibliography |
Related products
-
BSI PD CEN/TS 15634-2:2012
Foodstuffs. Detection of food allergens by molecular biological methods – Celery (Apium graveolens). Qualitative determination…
-
BS EN 15634-3:2023
Foodstuffs. Detection of food allergens by molecular biological methods – Hazelnut (Corylus avellana). Qualitative detection…
-
BS EN 15634-4:2023
Foodstuffs. Detection of food allergens by molecular biological methods – Peanut (Arachis hypogaea). Qualitative detection…
-
IEEE 802.3-2022
IEEE Standard for Ethernet Published By Publication Date Number of Pages IEEE 2022
-
IEEE 802.3-2018
IEEE Standard for Ethernet (Superseded Redline) Published By Publication Date Number of Pages IEEE 2018
-
IEEE 802.3-2005
IEEE Standard for Information Technology – Telecommunications and Information Exchange Between Systems – Local and…
-
BSI PD CEN/TS 15633-2:2013
Foodstuffs. Detection of food allergens by immunological methods – Quantitative determination of hazelnut with an…
-
BSI PD CEN/TS 15634-5:2016
Foodstuffs. Detection of food allergens by molecular biological methods – Mustard (Sinapis alba) and soya…
-
BSI PD CEN/TS 16634:2014
Personal identification. Recommendations for using biometrics in European Automated Border Control Published By Publication Date…
-
IEEE 802.3-2022(Redline)
IEEE Standard for Ethernet (Redline) Published By Publication Date Number of Pages IEEE 2022 7025
-
BS EN 15634-5:2023
Foodstuffs. Detection of food allergens by molecular biological methods – Mustard (Sinapis alba) and soya…
-
BS EN 15634-2:2019
Foodstuffs. Detection of food allergens by molecular biological methods – Celery (Apium graveolens). Detection of…
-
BSI PD CEN/TS 15634-4:2016
Foodstuffs. Detection of food allergens by molecular biological methods – Peanut (Arachis hypogaea). Qualitative detection…
