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BSI PD CEN/TS 17718:2022

BSI PD CEN/TS 17718:2022

$102.76

Plant biostimulants. Determination of Rhizobium spp.

Published By Publication Date Number of Pages
BSI 2022 18
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This document provides the methodology for the enumeration and determination of Rhizobium sp., Mesorhizobium sp., Ensifer sp., or Bradyrhizobium sp. in plant biostimulant products in accordance with the Regulation (EU) 2019/1009 of the European Parliament and of the Council [1].

PDF Catalog

PDF Pages PDF Title
2 National foreword
5 European foreword
6 Introduction
7 1 Scope
2 Normative references
3 Terms and definitions
8 4 Enumeration of Rhizobium sp., Mesorhizobium sp., Ensifer sp., Bradyrhizobium sp.
4.1 General
4.2 Sample preparation
4.2.1 General
4.2.2 Liquid – water-based formulations
4.2.3 Liquid – oil-based, emulsifiable concentrate (EC) formulations
9 4.2.4 Solid – wettable powder (WP) formulations
4.2.5 Solid – water dispersible granules (WDG) formulations
4.2.6 Solid – pellets, granules, microgranules – slow release – formulations
4.2.7 Solid – substrate
4.3 Serial dilution
10 4.4 Plate counts of rhizobia in sterile diluent
4.5 Spread-plate counting
11 4.6 Growth Media
4.7 Calculation
5 Species determination of Rhizobium sp., Mesorhizobium sp., Ensifer sp., Bradyrhizobium sp. via genetic analysis
5.1 General
5.2 Preparation of the sample for the genomic DNA extraction
5.2.1 Isolation and preparation of the microorganism
12 5.2.2 Sample concentration
5.2.3 DNA extraction and storage
5.2.4 Partial PCR Amplification of the 16S rRNA Genes
14 Annex A (informative) Formula of culture media
17 Bibliography

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BSI PD CEN/TS 17718:2022
$102.76