{"id":338542,"date":"2024-10-19T23:41:29","date_gmt":"2024-10-19T23:41:29","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-15634-12019\/"},"modified":"2024-10-25T22:47:39","modified_gmt":"2024-10-25T22:47:39","slug":"bs-en-15634-12019","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-15634-12019\/","title":{"rendered":"BS EN 15634-1:2019"},"content":{"rendered":"<p>This document provides the overall framework for detection of sequences corresponding to species containing allergens using the polymerase chain reaction (PCR). It relates to the requirements for the specific amplification of target nucleic acid sequences (DNA) and for the confirmation of the identity of the amplified nucleic acid sequence.<\/p>\n<p>Guidelines, minimum requirements and performance criteria laid down in European Standards are intended to ensure that comparable and reproducible results are obtained in different laboratories. This document has been established for food matrices.<\/p>\n<p>This document is intended to be used in addition to <span class=\"std\"><span class=\"std-ref\">EN&nbsp;15842<\/span><\/span>.<\/p>\n<h4>PDF Catalog<\/h4>\n<table border=\"1px\" class=\"des-table\">\n<tr>\n<th>PDF Pages<\/th>\n<th>PDF Title<\/th>\n<\/tr>\n<tr>\n<td><b>2<b><\/td>\n<td>National foreword  <\/td>\n<\/tr>\n<tr>\n<td><b>6<b><\/td>\n<td>European foreword  <\/td>\n<\/tr>\n<tr>\n<td><b>7<b><\/td>\n<td>Introduction  <\/td>\n<\/tr>\n<tr>\n<td><b>8<b><\/td>\n<td>1 Scope <br \/> 2 Normative references <br \/> 3 Terms and definitions <br \/> 3.1 Terms relative to extraction and purification of DNA  <\/td>\n<\/tr>\n<tr>\n<td><b>9<b><\/td>\n<td>3.2 Terms relative to amplification of DNA <br \/> 3.3 Definitions referring to controls  <\/td>\n<\/tr>\n<tr>\n<td><b>10<b><\/td>\n<td>4 General requirements for laboratories <br \/> 4.1 General <br \/> 4.2 Laboratory organization <br \/> 4.3 Apparatus and equipment <br \/> 4.4 Material and reagents  <\/td>\n<\/tr>\n<tr>\n<td><b>11<b><\/td>\n<td>5 Procedure <br \/> 5.1 Principle <br \/> 5.2 General <br \/> 6 Isolation\/Extraction of nucleic acid <br \/> 6.1 Preparation of sample <br \/> 6.2 DNA extraction <br \/> 6.3 DNA quantitation  <\/td>\n<\/tr>\n<tr>\n<td><b>12<b><\/td>\n<td>6.4 Quality of DNA <br \/> 6.5 Storage conditions of extracted DNA <br \/> 7 Amplification of specific target sequences <br \/> 7.1 Reagents <br \/> 7.1.1 Target DNA <br \/> 7.1.2 Water <br \/> 7.1.3 Deoxynucleoside triphosphate (dNTPs) solution <br \/> 7.1.4 PCR buffer solution  <\/td>\n<\/tr>\n<tr>\n<td><b>13<b><\/td>\n<td>7.1.5 Thermostable DNA Polymerase <br \/> 7.1.6 Optional additional reagents <br \/> 7.2 Primers <br \/> 7.2.1 Primer design <br \/> 7.2.2 Forward and reverse primer <br \/> 7.2.3 Validation of primer sequences  <\/td>\n<\/tr>\n<tr>\n<td><b>14<b><\/td>\n<td>7.3 PCR procedure <br \/> 7.3.1 General <br \/> 7.3.2 PCR profile and performance criteria <br \/> 7.3.3 PCR target descriptions <br \/> 7.3.4 Controls <br \/> 7.3.5 PCR amplification  <\/td>\n<\/tr>\n<tr>\n<td><b>15<b><\/td>\n<td>8 Detection and confirmation of PCR products <br \/> 8.1 General <br \/> 8.2 Quantitation of PCR products <br \/> 8.2.1 General considerations <br \/> 8.2.2 Specific considerations <br \/> 9 Interpretation  <\/td>\n<\/tr>\n<tr>\n<td><b>16<b><\/td>\n<td>10 Expression of the results <br \/> 10.1 General <br \/> 10.2 Expression of a positive result <br \/> 10.2.1 Qualitative PCR <br \/> 10.2.2 Quantitative PCR <br \/> 10.3 Expression of a negative result  <\/td>\n<\/tr>\n<tr>\n<td><b>17<b><\/td>\n<td>11 Test report  <\/td>\n<\/tr>\n<tr>\n<td><b>18<b><\/td>\n<td>Bibliography  <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p><b>Foodstuffs. Detection of food allergens by molecular biological methods &#8211; General considerations<\/b><\/p>\n<table class=\"shortdes-table\" style=\"width: 100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>Published By<\/td>\n<td>Publication Date<\/td>\n<td>Number of Pages<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/pdfstandards.shop\/product-category\/publishers\/bsi\/\"><b>BSI<\/b><\/a><\/td>\n<td>2019<\/td>\n<td>20<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":338545,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[124,2641],"product_tag":[],"class_list":{"0":"post-338542","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-30","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/338542","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/338545"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=338542"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=338542"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=338542"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}