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BS EN 16868:2019

BS EN 16868:2019

$167.15

Ambient air. Sampling and analysis of airborne pollen grains and fungal spores for networks related to allergy. Volumetric Hirst method

Published By Publication Date Number of Pages
BSI 2019 42
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This document specifies the procedure to sample continuously and to analyse the concentration of airborne pollen grains and fungal spores in ambient air using the volumetric Hirst type sampler [1] [2] [3] (see Annex A) or an even equivalent method assuring comparable data.

This document describes both the sampling and the analysis procedures for the purpose of networks related to allergy. For the other tasks mentioned in the introduction, other specifications may be required.

PDF Catalog

PDF Pages PDF Title
2 undefined
8 1 Scope
2 Normative references
3 Terms and definitions
12 4 Principle
5 Sampling
5.1 Equipment
5.1.1 Apparatus
16 5.1.2 Sampling support
5.1.2.1 General
17 5.1.2.2 Transparent tape
5.1.2.3 Reagents
18 5.1.3 Installation conditions
5.2 Operating procedure
5.2.1 Preparation of the coating medium
19 5.2.2 Support preparation
20 5.2.3 Changing of the drum
6 Analysis
6.1 Equipment
21 6.2 Operating procedure
6.2.1 Support
6.2.1.1 Microscope glass slide
6.2.1.2 Microscope cover glass
6.2.2 Mounting medium
6.3 Methodology for counting
6.3.1 Glass slide preparation for microscopy analysis for drum tape
23 6.3.2 Optical microscopy
24 6.3.3 Identification
6.3.4 Counting method
6.3.5 Data recording
25 6.3.6 Conversion factor
26 7 Performance characteristics for pollen and fungal spores counts
7.1 General
7.2 Integrated uncertainty assessment
7.3 Uncertainty from counting error and counting routine
7.4 Measurement uncertainty relating to sampling efficiency
7.5 Measurement uncertainty relating to capture film, adhesive and specimen preparation
27 7.6 Measurement uncertainty relating to time discrimination
7.7 Measurement uncertainty related to the detection limit
7.8 Measurement uncertainty in relation to the calibration of the flow rate
7.9 Measurement uncertainty relating to spatial representativity
8 Quality assurance
8.1 General
8.2 Measurement site/trap
8.2.1 Control
8.2.2 Characterization of the site and its ambient conditions (passport of sampling site)
28 8.2.3 Spatial representativity
8.3 Analyst
8.4 Intra- and interlaboratory quality assessments
8.4.1 General
8.4.2 Repeatability
8.4.3 Reproducibility and accuracy
29 8.4.4 Sensitivity and specificity
8.5 Network monitoring management
30 Annex A (informative)Hirst type volumetric trap
31 Annex B (informative)Pictures of impaction support
33 Annex C (informative)Material Safety Data Sheets
34 Annex D (informative)Identification key

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BS EN 16868:2019
$167.15