🔥🔥 Don’t Miss Out on Our End of Autumn Sale. If you have any questions, feel free to click the bottom right corner to contact our customer service..

Concat us[email protected]
Shopping Cart

No products in the cart.

🔍
BSI PD CEN/TS 17722:2022

BSI PD CEN/TS 17722:2022

$167.15

Plant biostimulants. Determination of mycorrhizal fungi

Published By Publication Date Number of Pages
BSI 2022 44
PDF Format Searchable Printable Preview Now
Guaranteed Safe Checkout
Category:

If you have any questions, feel free to reach out to our online customer service team by clicking on the bottom right corner. We’re here to assist you 24/7.
Email:[email protected]

This document was developed to provide a horizontal method for enumeration and genera/species determination [1], [2], [3] of mycorrhizal fungi in plant biostimulants products in accordance with the EU Fertilising Products Regulation.

PDF Catalog

PDF Pages PDF Title
2 undefined
9 1 Scope
2 Normative references
3 Terms and definitions
11 4 Methods for the quantification of mycorrhiza
4.1 General
13 4.2 How to prepare the initial sample
4.2.1 General
4.2.2 Liquid – water-based formulations
4.2.3 Liquid – oil-based (emulsifiable concentrate EC) formulations
4.2.4 Solid – wettable powder (WP) formulations
4.2.5 Solid – water dispersible granules (WDG) formulations
14 4.2.6 Solid – pellets, granules, microgranules (slow release) formulations
4.2.7 Solid – substrate
4.3 Enumeration methods
4.3.1 General
4.3.2 Method N° 1: Spore isolation and counting MTT
4.3.2.1 Procedure for enumeration of spores
15 4.3.2.2 Viability of spores
16 4.3.3 Method N° 2: Procedure for clearing and staining root samples and enumeration of vesicles in the stained root samples
4.3.3.1 Materials and equipment
17 4.3.3.2 Procedure
19 4.3.4 Enumeration of the total number of UPM in the product using Method N°1 + Method N°2
4.3.5 Method N°3: Endomycorrhiza bioassay
4.3.5.1 Principle of the MPN (Most Probable Number of mycorrhizal propagule)
24 4.3.5.2 Protocols and examples
27 4.3.6 Method N°4: Ectomycorrhiza and ericoid count
4.3.6.1 General
4.3.6.2 Sample preparation
4.3.6.3 Serial dilution
4.3.6.4 Plate counts of ectomycorrhiza in sterile diluent
28 4.3.6.5 Spread-plate counting with modified Melin Norkrans Medium (MNM) agar
4.3.6.6 Calculation
31 5 Molecular characterization and identification of mycorrhiza isolates
5.1 General
5.2 Materials and equipment
5.3 Method for the molecular characterization and identification of mycorrhiza isolates
5.3.1 Spores cleaning
32 5.3.2 DNA extraction
33 5.3.3 Preparation for PCR
5.3.3.1 Primer sequences
5.3.3.2 Master mix preparation
34 5.3.3.3 Procedure for running PCR
5.3.3.4 PCR program for each primer set
35 5.3.4 Preparation for gel-electrophoresis
5.3.4.1 Materials and equipment
5.3.4.2 Procedure
36 5.3.5 Direct sequencing (outsourced sequencing lab)
6 Method of molecular characterization and identification for ectomycorrhiza and ericoid
6.1 General
37 6.2 Materials
6.2.1 Fungal material
6.2.2 Molecular biology kits/chemicals
38 6.2.3 Equipment
6.3 Detailed description of method
6.3.1 Material preparation
39 6.3.2 DNA extraction and quality check
6.3.3 PCR amplification of ITS sequences
40 6.3.4 Gel electrophoresis and PCR product visualization

Related products

BSI PD CEN/TS 17722:2022
$167.15