{"id":389371,"date":"2024-10-20T03:49:43","date_gmt":"2024-10-20T03:49:43","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-157882021\/"},"modified":"2024-10-26T07:01:37","modified_gmt":"2024-10-26T07:01:37","slug":"bs-en-157882021","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-157882021\/","title":{"rendered":"BS EN 15788:2021"},"content":{"rendered":"
This document specifies general rules for the enumeration of enterococci (E. faecium) in feeding stuffs (additives, premixtures and compound feeds excluding mineral feeds) that contain enterococci as a single microorganism component or in a mixture with other microorganisms. Applying the method to premixtures and compound feeds with critical amounts of copper demands a special procedure (see A.2). The document is not applicable to mineral feeds which are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40 % crude ash (Regulation (EC) 767\/2009) [4]. There are different categories of feed samples: a) Additives containing about 1010 colony forming units (CFU)\/g; b) Premixtures containing 1011 CFU\/kg; c) Compound feeds, meal or pellets which contain about 109 CFU\/kg.<\/p>\n
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2<\/td>\n | undefined <\/td>\n<\/tr>\n | ||||||
8<\/td>\n | 1 Scope 2 Normative references 3 Terms and definitions 4 Principle <\/td>\n<\/tr>\n | ||||||
9<\/td>\n | 5 Diluents and culture media 5.1 Diluents <\/td>\n<\/tr>\n | ||||||
10<\/td>\n | 5.2 Culture media <\/td>\n<\/tr>\n | ||||||
12<\/td>\n | 6 Apparatus <\/td>\n<\/tr>\n | ||||||
13<\/td>\n | 7 Sampling 8 Preparation of the test sample 9 Procedure 9.1 Preparation of poured agar plates for spread plate method <\/td>\n<\/tr>\n | ||||||
14<\/td>\n | 9.2 Preparation of culture media for pour plate method 9.3 Preparation of the initial suspension and decimal dilutions <\/td>\n<\/tr>\n | ||||||
15<\/td>\n | 9.4 Inoculation and incubation of the plates 9.4.1 General 9.4.2 Spread plate method <\/td>\n<\/tr>\n | ||||||
16<\/td>\n | 9.4.3 Pour plate method 9.5 Enumeration of colonies 9.6 Confirmation <\/td>\n<\/tr>\n | ||||||
17<\/td>\n | 10 Expression of results 11 Precision 11.1 General 11.2 Interlaboratory studies <\/td>\n<\/tr>\n | ||||||
18<\/td>\n | 11.3 Repeatability 11.4 Reproducibility 12 Test report <\/td>\n<\/tr>\n | ||||||
19<\/td>\n | Annex A (informative)Notes on the procedure A.1 General A.2 Critical copper concentration <\/td>\n<\/tr>\n | ||||||
20<\/td>\n | Annex B (informative)Results of the interlaboratory studies B.1 Interlaboratory study based on bile aesculin azide agar B.2 Interlaboratory study based on Slanetz and Bartley agar <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Animal feeding stuffs: Methods of sampling and analysis – Detection and enumeration of Enterococcus (E. faecium) spp. used as feed additive<\/b><\/p>\n |