{"id":402594,"date":"2024-10-20T05:01:56","date_gmt":"2024-10-20T05:01:56","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-pd-cen-ts-177132022\/"},"modified":"2024-10-26T08:57:38","modified_gmt":"2024-10-26T08:57:38","slug":"bsi-pd-cen-ts-177132022","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-pd-cen-ts-177132022\/","title":{"rendered":"BSI PD CEN\/TS 17713:2022"},"content":{"rendered":"

This document provides the methodology for the enumeration and determination of Azospirillum spp. in plant biostimulant products in accordance to the Regulation of EU fertilising products [1].<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nNational foreword <\/td>\n<\/tr>\n
5<\/td>\nEuropean foreword <\/td>\n<\/tr>\n
6<\/td>\nIntroduction <\/td>\n<\/tr>\n
7<\/td>\n1 Scope
2 Normative references
3 Terms and definitions
4 Enumeration of Azospirillum spp.
4.1 General
4.2 Sampling
4.3 Preparation of the sample
4.3.1 Sample preparation <\/td>\n<\/tr>\n
8<\/td>\n4.3.2 Liquid -based water- formulations
4.3.3 Liquid \u2013 oil based emulsifiable concentrate (EC) formulations
4.3.4 Solid – Wettable Powder (WP) formulations
4.3.5 Solid – Water dispersible granules (WDG) formulations
4.3.6 Solid \u2013 Pellets, granules, microgranules (slow release) formulations
4.3.7 Solid – substrate
4.4 Requirements (apparatus) <\/td>\n<\/tr>\n
9<\/td>\n4.5 Preparation of the culture media
4.6 Preparation of serial dilution for MPN count
4.7 Incubation of tubes
4.8 Counting
4.9 Method for estimating MPN count <\/td>\n<\/tr>\n
10<\/td>\n5 Species determination of Azospirillum spp. via genetic analysis
5.1 General
5.2 Preparation of the sample for the genomic DNA extraction
5.2.1 Isolation and preparation of the microorganism
5.2.2 Sample concentration
5.2.3 DNA extraction and storage
5.2.4 Partial PCR amplification of the 16S rRNA genes <\/td>\n<\/tr>\n
12<\/td>\nAnnex A (normative) Formula of the culture media <\/td>\n<\/tr>\n
14<\/td>\nAnnex B (normative) Table of MPN values <\/td>\n<\/tr>\n
16<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Plant biostimulants. Determination of Azospirillum spp.<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2022<\/td>\n18<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":402602,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-402594","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/402594","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/402602"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=402594"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=402594"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=402594"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}